| Time | Status | Match | FT | H1 | Stats |
| 29/08/2016 16:00 |
(FT) | Boreham Wood[10] Maidstone United[12] |
0 1 |
0 0 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Forest Green Rovers[6] Southport FC[22] |
5 1 |
2 0 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | North Ferriby United[15] Barrow[14] |
0 1 |
0 0 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Solihull Moors[5] Macclesfield Town[9] |
2 3 |
2 1 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Sutton United[3] Dagenham and Redbridge[2] |
1 0 |
1 0 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Woking[23] Chester FC[19] |
3 1 |
2 1 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Wrexham[11] York City[18] |
2 1 |
1 1 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Braintree Town Torquay United |
1 3 |
1 2 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Bromley Eastleigh |
0 5 |
0 2 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Dover Athletic Aldershot Town |
1 2 |
1 2 |
MF | ME |
| 29/08/2016 16:00 |
(FT) | Lincoln City Gateshead |
3 0 |
1 0 |
MF | ME |
| 29/08/2016 18:30 |
(FT) | Tranmere Rovers Guiseley |
1 0 |
1 0 |
MF | ME |