| Time | Status | Match | FT | H1 | Stats |
| 17/09/2016 13:15 |
(FT) | Sutton United[15] Tranmere Rovers[3] |
1 0 |
0 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Chester FC[18] Braintree Town[22] |
1 0 |
1 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Dagenham and Redbridge[4] North Ferriby United[20] |
2 0 |
1 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Forest Green Rovers[2] Bromley[19] |
1 0 |
0 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Gateshead[7] Solihull Moors[17] |
0 0 |
0 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Maidstone United[12] Guiseley[24] |
1 1 |
1 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | York City[16] Dover Athletic[8] |
0 1 |
0 1 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Boreham Wood Torquay United |
2 0 |
2 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Lincoln City Barrow |
1 2 |
1 1 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Macclesfield Town Eastleigh |
0 1 |
0 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Southport FC Aldershot Town |
1 1 |
0 0 |
MF | ME |
| 17/09/2016 16:00 |
(FT) | Woking Wrexham |
2 0 |
0 0 |
MF | ME |